Home
Products
Supplements for Cell Culture
Slide 1 of 2
1
of
2
Cell culture supplements enable labs to customize and augment media formulations to suit specific cell culture applications such as protein production, hybridoma production, CHO (Chinese hamster ovary) cell applications, other serum-free workflows, and more. The cell culture- and insect cell culture-tested supplements collected here have undergone application testing for suitability in cell culture systems. This testing eliminates theneed for labs to screen these supplements prior to use in a cell culture application. ITS Supplements Hybridoma Reagents Amino Acids, Antibiotics, and Other Cell Culture Supplements Popular CategoriesRead more about:
Serum-free and reduced-serum cell culture media formulations often require supplementation with the factors normally present in serum that are required for healthy culture growth. ITS reagent is used as a basal supplement when the goal is to reduce or eliminate the use of fetal bovine serum (FBS) in culture media, when culture media must be animal origin-free, or to tailor nutrients and their concentrations for specific cell culture applications. ITS is named for the constituent nutrients insulin, transferrin, and selenium. Variants on this nutrient co*cktail include SITE (selenium, insulin, transferrin, ethanolamine), and enhanced formulations that may also incorporate linoleic acid, oleic acid, and/or bovine serum albumin (BSA).ITS Supplements
The biological function of key constituent ingredients in ITS and its variants are as follows: Traditional methods for monoclonal antibody production have relied upon the use of medium supplemented with serum of animal origin. When these antibodies are employed for research or in vitro diagnostic applications, the presence of serum does not pose significant regulatory concerns, and efforts to develop serum-free media have focused primarily on cost reduction. As antibodies are increasingly used as biotherapeutics, the need for media and reagents that ensure robust hybridoma culture without the use of sera and other animal products has increased. Our Hybri-Max™ supplements are culture-tested for optimal performance in hybridoma culture. These and other hybridoma-designated supplements support robust culture in monoclonal antibody labs. Our cell culture-tested amino acid supplements include both polyamine reagents and individual amino acids that enable custom enrichment of cell culture media formulations. Our complete culture supplement solutions include cholesterol, lactoferrin, linoleic acid, glucagon, cyclodextrin, yeast extracts, antioxidants, vitamins, antibiotics, and more.Hybridoma Reagents
Amino Acids, Antibiotics, and Other Cell Culture Supplements
Synthetic
Human
Porcine
Bovine
Ovine
Related Product Resources
- Cell Culture Manual
- /language-masters/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/the-cell-environment
Related Product Categories
Choose from a range of optimized cell culture media formulations, including DMEM, RPMI-1640, and serum-free options for diverse cellular needs.
Mammalian Cell Lines
Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.
Stem Cells
Our complete range of ES and iPS cell-qualified products includes stem cell lines and media for differentiation and maturation, and provides stem cell researchers with convenient and cost-effective solutions for the reliable culture of pluripotent stem cells.
Primary Cells
Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.
Attachment Factors
Attachment factors mimicking the extracellular matrix (ECM) provide important cues to cultured cells that stimulate physiological growth and behavior. A wide array of biological and synthetic polymers can be selected to enhance cell culture conditions.
Hormones
Boost cell growth using essential hormones: insulin, dexamethasone, β-Estradiol, and others. Elevate your cell culture success.
Amino Acids
We have a comprehensive offering of high-purity amino acids and derivatives important to metabolomic pathways and protein structure for your research needs.
Related Applications
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Primary Cell Culture Techniques
Cultivate mammalian primary cells for ADME/Tox screening, maintaining physiological relevance for accurate results.
Stem Cell Culture
An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
Cell-Based Assays
Cellular assays, or cell-based assays, are essential tools for measuring cell health, viability, proliferation, migration, invasion, chemotaxis, apoptosis, angiogenesis, oxidative stress, cell signaling and live cell analysis.
